Volume 25, Issue 115, September 2021

Protective effect of some food supplement against the toxicity of cyclophosphamide on the brain

Ali Hassan A Ali^1,2♦, Shaban Ragab Ibrahim^3,4, Abdulrahman M Alkassar Alanazi⁵, Faisal M Alshmmari⁵, Abduallah A Alotaibi⁵, Abdulmohsen K Alghamdi⁵

¹Anatomy Department, College of Medicine, Prince Sattam Bin Abdulaziz University, Alkharj 11942, KSA
²Anatomy Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt
³Forensic Medicine and Toxicology Department, Prince Sattam Ibn Abdelaziz University, Saudi Arabia Alkharj 11942, KSA
⁴Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt
⁵College of Medicine, Prince Sattam Bin Abdulaziz University, Al-Kharj, KSA

^♦Corresponding author
Anatomy Department, College of Medicine, Prince Sattam Bin Abdulaziz University, Alkharj 11942, KSA / Anatomy Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt

ABSTRACT

The nervous system had been found to be affected by oxidative stress. Natural dietary supplements had been proved that they have antioxidants properties that may protect our bodies against the effects of free radicals. Aphanizomenon flos-aquae (AFA) are a dietary supplement with scientifically demonstrated health-improving effects especially on the nervous system. Cyclophosphamide (CP) is a widely used medication in chemotherapy and can cause oxidative stress. This study was conducted to investigate the role of AFA in preventing cyclophosphamide-induced adverse effects on the brain tissue of CP-treated rats. It is an experimental study carried out in the period from November 2020 to June 2021. It was performed on 30 albino rats with body weights of 280-320 g. The animals were divided into the following three groups. Group 1 (control group), Group 2 (CP group, received a single dose of CP at 100 mg/kg-1 BW intraperitoneally), and Group 3 (CP+ AFA, received orally extract of AFA for 30 days after CP injection). The morphological and histological structures of the brain were compared in the different groups of rats. Paraffin sections were prepared for histological, histochemical, immune-histochemical and morphometric studies. The data were statistically analyzed. Examined sections showed significant cellular injury in group 2 in comparison to the control groups. Group 3 showed marked improvements in the changes that occurred compared to the second group. These results provide evidence that AFA has a protective effect as they reduced the pathological cellular injuries in the cerebral cortex cells induced by cyclophosphamide.

Keywords: Antioxidant, Cyclophosphamide, Rat, Brain, AFA, Food Supplement

Medical Science, 2021, 25(115), 2411-2419

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