Volume 25, Issue 112, June 2021

Vascular Endothelial Growth Factor (VEGF) as biological markers expressed in ovarian cancer using immuno-histochemical technique

Misaa Omer Mohamed¹, Elsadig Ahmed Adam¹, Banaga Mnsoor Abdlla², Sara Abdelghani³, Lienda Bashier Eltayeb^4♦

¹Department of Histopathology and cytology, Faculty of Medical Laboratory Sciences, Al- Neelain University, Khartoum, Sudan
²Department of clinical chemistry, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan
³Department of parasitology, Faculty of Medical Laboratory Sciences, AlNeelain University, Khartoum, Sudan ⁴Department of Medical Laboratory Sciences, College of Applied medical sciences, Prince Sattam Bin Abdul-Aziz University, Al-Kharj, Riyadh, KSA

^♦Corresponding author
Department of Medical Laboratory Sciences, College of Applied medical sciences, Prince Sattam Bin Abdul-Aziz University, Al-Kharj, Riyadh, KSA

ABSTRACT

Background: Vascular endothelial growth factor (VEGF) has vital role in tumor metastasis through triggering the accumulation of abnormal blood vessels. Aim: The study aimed to evaluate the expression of VEGF in ovarian cancer in Sudanese patients to assess the correlation between expression of this marker and patient's age. Methods: A vascular endothelial growth factor expression was detected by immune-histochemical method on total of 38 Paraffin blocks of which 24 cases previously diagnosed as serous carcinoma and 14 cases mucinous carcinoma using routine Hematoxylin and Eosin stain. Result: Using 38 (FFPE) block of ovarian tumor; histopathological diagnosis of samples revealed that 14(37%) of samples were mucinous, 24(63%) samples were serous carcinoma. Patients ages ranged between 26-70 years, majority of them (42.1%) were in age group 47-58 years old. The VEGF marker were expressed in 29 ovarian paraffin wax embedded blocks, 19(50%) positive in SOC, while MOC 10(26.3 %) were positive the result statistically insignificant (P-value 0.855). 9(23.76%) were positive in age group (26-36), and 12(31.5%) were positive in age group (48-58) the result statistically significant p-value (0.044). Conclusion: There is statistically significant association between VEFG expression and advance age, while didn’t correlated with histological types of ovarian cancer. Further study should be done involving large sample size of ovarian cancer using more advance technique such as ovarian cancer Vm RNA testing.

Keywords: Ovarian cancer, VEFG, Immuno-histochemical stain

Medical Science, 2021, 25(112), 1311-1317

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