Background: The development of antibiotic resistance is an emergent global issue and a major threat to community health safety in
the 21st century. The threat of a ‘post-antimicrobial age’ where current antimicrobial agents will be ineffective due to increasing
levels of resistance has become a concern to all healthcare associations. Objective: This study aimed to assess the knowledge,
attitude and practice towards antibiotic utilization among undergraduate students in a public university in Malaysia. Methodology:
This was a cross-sectional descriptive study, using a pre-validated self-administered questionnaire to collect the needed data from
students. All data were checked for completeness and accuracy and then coded for analysis. Descriptive statistics were used to
present the data on demographic characteristics, knowledge, attitudes, and practice towards antibiotic utilization. The Fishers exact
tests or Chi-square, Mann-Whitney test and Kruskal-Wallis test were used wherever appropriate to evaluate the relationship
between independent and dependent variables using a p-value < 0.05 as statistically significant. Results: The response rate of the
present study was 95.4%. It was found that half of the students had a poor level of knowledge on the proper use of antibiotics;
whereas 44.4% had moderate and only 5.6% of students had a good level of knowledge. More than 90% of the students assumed
that antibiotics can cure viral infections. The mass population (79%) had a positive attitude towards checking the expiry date and
more than 70% follow the instructions stated on the label. More than 50% of the students completed the prescribed antibiotic
course. Gender (p=0.002), race (p=0.014), family monthly income (p=0.006) and nationality (p=0.049) were the more significantly
associated factors with the knowledge of students. Conclusion: The current findings revealed an inadequate level of knowledge
regarding the rational use of antibiotics among students. There is a need for a well-planned, organized, and structured public health
campaigns to raise the awareness of the community on the detrimental consequences of overuse and misuse of antibiotics.
Keywords: Rational use of antibiotics; knowledge; attitude; practice; undergraduate students; Malaysia