Volume 24, Issue 105, September - October, 2020

Screening L-Glutaminase producing some Pseudomonas sp. isolated from contact lenses by Rapid Plate Assay

Al-Zahrani NH

Department of Biology Sciences, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia; Email: nhalzahrani@uj.edu.sa

ABSTRACT

L-glutaminase is enzyme led to decomposition glutamine to glutamic and ammonia in the presence of water. Twenty isolates were isolated from the eyes wearers’ lenses in Jeddah city-Saudi Arabia. The bacterial ability to produce L-glutaminase was detected by rapid plate method and assayed by agar well diffusion method. Based on16S rDNA sequencing, the highest L-glutaminase producer strain was submitted under accession numbers as (pseudomonas aeruginosa B17 KX963365.1). The maximum enzyme production was performed at 35 °C in a shaking incubator, at 7pH after 24 hours by pseudomonas aeruginosaB17, and was partially purified. The enzyme was precipitable at 80% ammonium sulphate sedimentation; it was thermally stable at 30 °C and 7.5 pH. SDS-PAGE of purify L-glutaminase given (1) band with molecular weight (138.761kb).

Keywords: pseudomonas aeruginosa, L-glutaminase, 16S rDNA

Medical Science, 2020, 24(105), 3647-3654

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