Volume 24, Issue 104, July - August, 2020

Activities of selected antioxidants in saliva and plasma in patients with periodontal diseases - initial results

Janka Kluknavská¹, Beáta Bolerázska¹, Silvia Timková¹, Janka Vašková^2♦

¹1st Department of Stomatology, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Tr. SNP 1, 040 11, Košice, Slovak Republic
²Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Tr. SNP 1, 040 11, Košice, Slovak Republic

^♦Corresponding author
Dr. Janka Vašková, PhD., Assoc. prof., Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Tr. SNP 1, 04066 Košice, Slovak Republic, Tel.: +421 552343232 E-mail: janka.vaskova@upjs.sk

ABSTRACT

Background: Periodontal diseases are very common, beginning with inflammation caused mostly by dental plaque bacteria. Persistent infection can lead to soft tissue damage and tooth loss. Oxidative stress is common in all oral inflammatory diseases and may affect systemic changes. Methods: The selected enzymes (superoxide dismutase, glutathione peroxidase and reductase) as well as non-enzyme antioxidant (reduced glutathione) parameters in saliva and plasma from patients with diagnosed gingivitis, a chronic and aggressive form of periodontitis were detected and compared to healthy individuals. Results: Initial results of the study come from analyses in a small number of patients, which is likely also the reason for the lack of statistical significance in the differences between parameters obtained in experimental groups and the control group. Even so, it is apparent that oxidative stress is indicative of changes in the activities of antioxidant enzymes (superoxide dismutase, glutathione peroxidase, glutathione reductase) relative to reduced glutathione concentrations. Conclusions: There are positive correlations between saliva and plasma superoxide dismutase, glutathione peroxidase and glutathione reductase activities. This would allow for the dissemination of results within research groups in favour of analyses of more readily available biological material from patients.

Keywords: antioxidant enzyme, antioxidant marker, parodontitis, periodontal disease, reactive oxygen species, saliva

Medical Science, 2020, 24(104), 2411-2417

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