Volume 24, Issue 104, July - August, 2020

Evaluation of hepatoprotective action of Solanum melongena l. peel extract against paracetamol induced liver damage in albino rats

Jaideep Sarkar^1♦, Nitin Kumar², Pankaj Gupta³

¹Research Scholar, School of Medical and Allied Sciences, K.R. Mangalam University, Sohna Road, Gurgaon, Haryana, Pin- 122003, India
²Professor and Dean, School of Medical and Allied Sciences, K.R. Mangalam University, Sohna Road, Gurgaon, Haryana, Pin- 122003, India
³Assistant Professor, School of Medical and Allied Sciences, K.R. Mangalam University, Sohna Road, Gurgaon, Haryana, Pin- 122003, India

^♦Corresponding author
Research Scholar, School of Medical and Allied Sciences, K.R. Mangalam University, Sohna Road, Gurgaon, Haryana, Pin- 122003, India; Email: sombhu89@gmail.com

ABSTRACT

Introduction: Paracetamol or also called as acetaminophen or APAP is known from long time to induce hepatotoxicity in human and as well as experimental animals. Free radical injury and oxidative stress can be accounted for pathogenesis and progression of hepatic toxicity. In this context out of many naturally reported anti-oxidants, Solanum melongena is known by many common names; to name a few as egg plant or brinjal was selected due to its high anti-oxidant potential, easy palatability and also potential to act as functional food. From ethnobotanical claims, it’s reported to cure degenerative ailments through its strong anti-oxidant potential. Materials and methods: Extraction of S. melongena L. purple fruit peels were conducted in water: ethanolic mixture (1:1) and aqueous media to get SMHA and SMAQ, respectively. Following OECD test guideline 423, acute oral toxicity was conducted in Swiss Webster mice with SMHA and SMAQ at75, 150, 250, 500, 1000, and 2000 mg/kg/bw. There were no signs of evident toxicity in mice. So, 100, 200, and 400 mg/kg of SMHA and SMAQ was selected and orally administered to PCM induced hepatotoxic Wistar rats. Serum bio-chemical estimation (fasting sugar, AST, ALT, ALP, TC, TG, and HDL) was conducted after 24 hours post PCM treatment. Tissues were subjected to bio-chemical tests for estimating liversuperoxide dismutase (SOD), glutathione reductase (GSH) level. Microscopical examination was performed on liver cells. Results: In rats, serum and liver biomarker enzymes, LDL levels and elevated glucose levels were attenuated in dose dependent manner and were in normal ranges post treatment with SMHA and SMAQ when compared with Silymarin group rats. From histopathological observations, it was established that there was reduced fatty deposits or adipocytes infiltration in hepatocytes. This is a potential marker for recovery of hepatocytes against hepatotoxicity by PCM following lipid peroxidation mechanism. Statically all the findings concerning serum and tissue pathology was at p<0.05. Conclusion: The present study established that S. melongena L. possess excellent anti-oxidant potential and thereby subsequent hepato-protective activity too by attenuating free radicals and elevated liver biomarker enzymes. From functional food point of view, it can be used as both preventative as well as curative agent in liver compromised people. It evinced future potential for a new drug development for treating liver impairment.

Keywords: fruit peel, functional food, hepatoprotective, Nasunin, Solanum melongena L.

Medical Science, 2020, 24(104), 2013-2024

PDF