Volume 24, Issue 103, May - June, 2020

Immunohistochemical Analysis of CD34 and CD20 Markers Expression in Oral Squamous Cell Carcinoma

Babak Karimi¹, Mahsa Saidian^2♦, Fateme Zarouni³, Ali Rohani³, Parisa Boostanifard³, Ghaffar Barati³

¹Assistant professor, Faculty of dentistry, Department of Oral and Maxillofacial Pathology Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
²Dentist, Faculty of dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
³Dentist, Faculty of dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

^♦Corresponding author
Mahsa Saidian, Dentist, Faculty of dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

ABSTRACT

Background and objective: Oral squamous cell carcinoma (OSCC) is the most common oral malignancy manifests in oral cavity. The aim of the present study was to examine the expression of CD34 (vascular marker) and CD20 (pan B-cell marker) markers in Oral Squamous Cell Carcinoma using immunohistochemical analysis (IHC). Materials and methods: IHC staining was implemented on 15 paraffin-embedded OSCC samples with anti CD34 and CD20 markers antibodies. The peritumoral and intratumoral hot spot areas were designated separately for lymphatic density and blood vessel counting. Data were analyzed using t-test, ANOVA, and SPSS Ver. Results: The expression of CD34+ and CD20+ in the peritumoral and intratumoral compartments was statistically significant. No significant correlation was found between CD34+ and CD20+ cells in the peritumoral region. However, a significant correlation was found between B cells (B lymphocytes) and vascular density in the intratumoral area. The degree of B cell and vascular density proliferation in the intratumoral area was significantly more than peritumoral area. Conclusion: There is a positive correlation between CD20-positive B cell infiltration and expression of vascular endothelial growth factor. The severity of tumor angiogenesis promotes CD20+ B-cell infiltration.

Keywords: Oral squamous cell carcinoma, B-lymphocytes, Angiogenesis, CD34, CD20

Medical Science, 2020, 24(103), 1247-1254

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