Volume 16, Issue 37, January - June, 2022

Molecular docking study of potential phytochemicals of Tinospora cordifolia (Giloy) and Glycyrrhiza glabra (Mulethi) and their effect on the complex SARS-CoV2 Mpro& RdRp

Pandey J¹, Mishra SK²

¹Department of Biotechnology, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, Uttar Pradesh, India
²Department of Biotechnology, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, Uttar Pradesh, India

ABSTRACT

The present study is aimed at determining the total flavonoid and phenol contents and antioxidant properties of Silver nanoparticles (AgNPs) biosynthesized using unripe pawpaw peel (UPPAE) and banana peel (UBPAE) aqueous extracts. AgNPs of UPPAE and UBPAE were synthesized separately by reducing AgNO3 with UPPAE and UBPAE respectively. Primary characterization was done on the synthesized AgNPs with a UVvisible spectrophotometer, and their antioxidant properties were evaluated using DDPH, ABTS, FRAP assays. The reaction mixtures of AgNO3 solution and the agro-waste extracts turned dark brown for AgNO3 + UPPAE and light brown for AgNO3 + UBPAE and also displayed a UV-visible spectrum of 450 nm and 421 nm respectively which is characteristic of silver nanoparticles. The result of the total flavonoid contents revealed that AgNPs-UPPAE had the highest amount of flavonoid content (499.89 ± 1.96 mgQE/g) and phenol content (85.30 ± 0.57 mgGAE/g). However, the antioxidant result revealed that AgNPs-UBPAE displayed the highest DPPH scavenging activity with an IC50 value of 52.98 µg/ml when compared to AgNPs-UPPAE (52.98 µg/ml) and Ascorbic acid (59.93 µg/ml). AgNPs-UPPAE and AgNPs-UBPAE showed maximum ABTs scavenging activities with IC50 values of 52.20 µg/ml and 52.45 µg/ml respectively which is comparable to Ascorbic acid (50.95 µg/ml). The result of FRAP revealed that AgNPs-UPPAE and AgNPs-UBPAE had the highest FRAP value at a concentration of 25µg/ml unlike Ascorbic acid whose highest FRAP value was at 100 µg/ml. This result reveals the potential use of AgNPs-UPPAE and AgNPs-UBPAE as alternative natural antioxidants for the management of oxidative stress-induced ailments.

Keywords: Drug discovery, Molecular docking, SARS-CoV-2, Plants-derived phytochemicals, main protease, RNA dependent RNA polymerase, Tinospora cordifolia, Glycyrrhiza glabra.

Drug Discovery, 2022, 16(37), 9-17

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