Volume 60, Issue 335, May - August 2024

Evaluation of haematological parameters, and liver profiles following the administration of leached water in male Wistar rats

Peter IU^1♦, Olatunbosun TH¹, Agogo MK², Akpan UP¹

¹Department of Medical Physiology, Faculty of Basic Medical Sciences, University of Uyo, Uyo, Nigeria
²Department of Medical Physiology, Faculty of Basic Medical Sciences, University of Cross River State (UNICROSS), Nigeria

^♦Corresponding Author
Department of Medical Physiology, Faculty of Basic Medical Sciences, University of Uyo, Uyo, Nigeria

ABSTRACT

Landfills are a significant threat to groundwater worldwide. Leachate is a liquid formed when water passes through waste materials. This study investigated the effect of leached water from the Uyo village road dumpsite on hematological parameters and liver profiles using male Wistar rats. Twenty male Wistar rats weighing 150g-180g were randomly assigned into four subgroups, each consisting of five rats. The control group received 10ml of distilled water, Group 2 received 10ml/kg body weight of the leached water, Group 3 received 20ml/kg body weight of the leached water, and Group 4 received 10ml/kg body weight of boiled/cooled leached water. The administration was done orally for 21 days. Serum was then obtained through centrifugation of the blood samples and used to determine RBC count, hemoglobin level, WBC count, AST, ALT, and ALP levels. Administration of leached water significantly reduced the levels of red blood cells, hemoglobin, and white blood cells, which implies that leached water harms blood indices. The increased levels of ALT, AST, and ALP in rats exposed to leached water imply that leachate can induce hepatic damage, possibly through necrosis caused by its toxic constituents. In conclusion, water bodies surrounding the Uyo village road dumpsite should be tested and treated before consumption, as contamination by leachate can lead to physiological dysfunctions.

Keywords: Leachate, landfill, hematological parameters, liver profiles

Discovery, 2024, 60, e20d1439

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DOI: https://doi.org/10.54905/disssi.v60i335.e20d1439

Published: 29 May 2024