Volume 59, Issue 327, March 2023

Impaired CD4⁺CD69⁺ cells activation associated with elevated IL-6 and IL-10 in Togolese SLE patients

Pélagie Edlom Tchadié^1,2♦, Gnatoulma Katawa¹, Manuel Ritter³, Christèle Nguepou Tchopba¹, Marthe Oukoe Amessoudji¹, Bayaki Saka⁴, Malewe Kolou⁴, Mounerou Salou⁴, Lamine Baba-Moussa⁵, Yaovi Ameyapoh¹, Owonayo Oniankitan⁴, Andre Bigot²

¹Ecole Supérieure des Techniques Biologiques et Alimentaires (ESTBA)/Laboratoire de Microbiologie et de Contrôle de Qualité des Denrées Alimentaires/Unité de Recherche en Immunologie et Immuno - modulation (UR2IM), Université de Lomé, Lomé, Togo
²Faculté des Sciences de la Santé (FSS), Université d’Abomey Calavi, Cotonou, Bénin
³Institute for Medical Microbiology, Immunology and Parasitology (IMMIP), University Hospital Bonn (UKB), Bonn, Germany
⁴Faculté des Sciences de la Santé (FSS), Université de Lomé, Lomé, Togo
⁵Faculté des Sciences et Techniques (FAST), Laboratoire de Biologie et Typage Moléculaire en Microbiologie, Université d’Abomey Calavi, Abomey Calavi, Bénin

^♦Corresponding author
Ecole Supérieure des Techniques Biologiques et Alimentaires (ESTBA)/Université de Lomé, BP1515, Lomé, Togo, Faculté des Sciences de la Santé (FSS), Université d’Abomey Calavi, Cotonou Bénin

ABSTRACT

Introduction: Systemic lupus erythematosus (SLE) is an autoimmune disease involving complex pathogenesis which remains poorly investigated in developing countries. Indeed, mononuclear blood cells and cytokines play key role in the pathogenesis of the disease. The aim of this study was to investigate the monocluear blood cells and their cytokines profile in togolese SLE patients. Methods: A cross-sectional study was performed on five SLE patients and seven healthy donors. Mononuclear blood cells were characterized by flow cytometry. Cytokines from plasma: IL-6, TNFα, IFNγ, IL-5, IL-10 and IL-17A were assayed by sandwich ELISA. Results: CD4⁺cells count was decreased with an impaired activation showed by low expression of activation marker CD69 in SLE patients compared to healthy donors. SLE patients were also characterized by high production of plasma IL-6 and IL-10. Conclusion: Togolese SLE patients are characterized by impaired CD4⁺CD69⁺ cells activation, associated with high plasma levels of IL-6 and IL-10.

Keywords: Systemic lupus erythematosus, mononuclear blood cells, cytokines, SLE patients, Togo

Discovery, 2023, 59, e33d1029

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Published: March 2023