Volume 28, Issue 154, December 2024

Anti-microbial effect of Saussurea costus on multidrug-resistant bacteria

Sally Elnawasany^1,2♦, Yusuf A Haggag³, Hoda S Nouh⁴

¹Department of Tropical Medicine, Tanta University, Tanta, Egypt
²Department of Clinical Science, Al Rayan Colleges, Al-Madina, KSA
³Department of Pharmaceutical Technology, Tanta University, Tanta, Egypt
⁴Department of Botany and Microbiology, Tanta University, Tanta, Egypt

^♦Corresponding Author
Department of Tropical Medicine, Tanta University, Tanta, Egypt, Department of Clinical Science, AL-Rayan Colleges, Al-Madina, KSA

ABSTRACT

Background and objective: Saussurea costus was widely used in traditional medicine to treat respiratory problems. Its anti-microbial effect was investigated in multiple studies. This work aims to examine the anti-microbial effect of ZnO-NPs of Saussurea costus on some respiratory bacteria. Methods: After preparation of methanolic extract of Saussurea costus roots, we synthesized zinc oxide nanoparticles (ZnO-NPs) using an aqueous extract from Saussurea costus. The anti-microbial effect against Klebsiella pneumonia, Staphylococcus aureus, Acinetobacter baumannii, and Pseudomonas aeruginosa and their reference strains were assessed through well plate agar diffusion method, minimal inhibitory concentration (MIC), and minimal bacterial concentration (MBC). Results: Both methanolic extract and Zn-ONPs exhibited anti-microbial activity with a variable diameter of inhibition zones, where the largest inhibition zone was observed against the standard strain A. baumannii ATCC 19606 followed by A. baumannii and K. pneumonia clinical isolates (20 ± 0.03, 16 ± 0.01, and 16 ± 0.00 mm, respectively). ZnO-NPs have MICs in the range of 3.75-7.5 mg/mL, while the MIC values of S. costus methanolic extract ranged from 3.75 mg/mL to 15 mg/mL. The MBC values were generally found to be twice the inhibitory concentrations. Conclusions: Saussurea costus methanolic extract and ZnO-NPs have an antimicrobial effect against multidrug-resistant bacteria.

Keywords: Saussurea costus, anti-bacterial effect, multidrug-resistant bacteria, nanoparticles

Medical Science, 2024, 28, e146ms3473

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DOI: https://doi.org/10.54905/disssi.v28i154.e146ms3473

Published: 03 December 2024