Volume 25, Issue 114, August 2021

Evaluation of anticancer activity of Paclitaxel (PLT) and D-L Sulfarophane (SFN) as natural vegetable derivative against colon cancer cell line: In vitro study

Ali Hassan A Ali^1,2♦, Abdulrahman M Almalki³, Talal A Alharbi³, Musaad H Alanzy³, Muath A Alghuwainem³, Fahd M Alanazi³, Nasser I Alshumaymiri³, Aly Fahmy Mohamed^4,5

¹Anatomy Department, College of Medicine, Prince Sattam Bin Abdulaziz University, Alkharj 11942, KSA
²Anatomy Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt
³College of Medicine, Prince Sattam Bin Abdulaziz University, Al-Kharj, KSA
⁴The International Center for Advanced Researches (ICTAR-Egypt), Cairo, Egypt
⁵Histology department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt

^♦Corresponding author
Anatomy Department, College of Medicine, Prince Sattam Bin Abdulaziz University, Alkharj 11942, KSA Anatomy Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt

ABSTRACT

Paclitaxel (PTx) and D-L Sulforaphane (SFN) natural derivatives could inhibit many types of cancerous cells proliferation through apoptosis and autophagy. Toxicity of PTx and SFN to Caco-2 cells was concentration dependent using MTT assay. Sole SFN was significantly toxic than sole PTx. PTx and SFN combination induced a synergetic potential of PTX toxicity than sole form and the recorded IC50 in μgm / ml was in the order of (862.8,2.,8 and 22.37) for sole PTx, SFN and combined form respectively. PTx and SFN sole and combined form induced Caco-2 cells DNA accumulation at the G2/M phase but not significantly at the Go/G1 and S phases. Apoptosis was proved via a significant up regulation of P53, Cy-c, Bax, casp-3 and down regulation of Bcl-2 with elevated Bax/Bcl-2 ratio. Antioxidant activity supported apoptotic potential showed significantly elevated ROS, MDA and reduced GSH.

Keywords: Anticancer, Paclitaxel, Sulfarophane, Cytotoxicity, Apoptosis, Flow cytometry, Cancer colon

Medical Science, 2021, 25(114), 1900-1909

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